myTXTL®: Breaking Out of the Cell to Enable High-Throughput Protein Engineering
Ben Steil
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Daicel Arbor Biosciences
Next generation sequencing and affordable, high quality DNA synthesis have enabled a new era of protein engineering. Novel proteins guided by both computational and natural design can now be generated quickly. Typically, protein expression is done in vivo, requiring days before obtaining purified protein suitable for analysis. This bottleneck slows down the desired high-throughput design-build-test-learn process. Increasingly, cell-free protein expression systems capable of transcription-translation (TXTL) have enabled researchers to remove this bottleneck allowing analysis of protein function within hours, not days. Cell-free systems are utilized in an increasing number of applications, including bacteriophage rebooting, gene circuits, microfluidics, and artificial cells.